# Experiment Protocol: Title

| Field | Value |
|-------|-------|
| Protocol ID | PROT-NNN |
| Version | N.N |
| Author | Name |
| Date | YYYY-MM-DD |
| Approval | PI / lab head |

## Objective & hypothesis

- **Objective**: what this protocol measures
- **Hypothesis**: $H_0$: ... vs $H_1$: ...

## Materials

| Reagent / item | Spec | Source | Storage |
|----------------|------|--------|---------|
| Item | Grade | Supplier | Condition |

## Safety

- Hazard + PPE required
- Waste disposal procedure
- Emergency contact / spill procedure

## Procedure

1. **Preparation** – preconditioning, calibration steps
2. **Step** – with parameter values and tolerances
3. **Step** – critical points marked `CRITICAL`
4. **Data capture** – what is recorded, at what frequency

## Controls

- Positive control:
- Negative control:
- Blanks:

## Data collection

| Variable | Unit | Instrument | Precision |
|----------|------|------------|-----------|
| var | unit | device | ±N |

Sample size justification:

$$
n = \frac{2\sigma^2 (z_{\alpha/2} + z_{\beta})^2}{\delta^2}
$$

## Analysis plan

- Statistical test to use and its assumptions
- Exclusion criteria defined *before* data collection

## Deviations

Any deviation from this protocol must be logged with reason and date.

## References

- Method source / prior protocol version