Experiment Protocol: Title

Field	Value
Protocol ID	PROT-NNN
Version	N.N
Author	Name
Date	YYYY-MM-DD
Approval	PI / lab head

Objective & hypothesis

• Objective: what this protocol measures
• Hypothesis: $H_0$: ... vs $H_1$: ...

Materials

Reagent / item	Spec	Source	Storage
Item	Grade	Supplier	Condition

Safety

• Hazard + PPE required
• Waste disposal procedure
• Emergency contact / spill procedure

Procedure

1. Preparation – preconditioning, calibration steps
2. Step – with parameter values and tolerances
3. Step – critical points marked CRITICAL
4. Data capture – what is recorded, at what frequency

Controls

• Positive control:
• Negative control:
• Blanks:

Data collection

Variable	Unit	Instrument	Precision
var	unit	device	±N

Sample size justification:

$$n = \frac{2\sigma^2 (z_{\alpha/2} + z_{\beta})^2}{\delta^2}$$

Analysis plan

• Statistical test to use and its assumptions
• Exclusion criteria defined before data collection

Deviations

Any deviation from this protocol must be logged with reason and date.

References

• Method source / prior protocol version